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KMID : 0364820180540040428
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Korean Journal of Microbiology
2018 Volume.54 No. 4 p.428 ~ p.435
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Antimicrobial activities of Burkholderia sp. strains and optimization of culture conditions
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Nam Young-Ho
Choi Ah-Young
Hwang Buyng-Su
Chung Eu-Jin
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Abstract
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In this study, we isolated and identified bacteria from freshwaterand soil collected from Osang reservoir, to screen antimicrobialbacteria against various pathogenic bacteria. 38 strains wereisolated and assigned to the class Proteobacteria (22 strains),Actinobacteria (7 strains), Bacteroidets (6 strains), and Firmicutes(3 strains) based on 16S rRNA gene sequence analysis. Amongthem, strain OS17 showed a good growth inhibition against 5methicillin-resistant Staphylococcus aureus subsp. aureus strainsand Bacillus cereus, Bacillus subtilis, Filobasidium neoformans. As a result of the 16S rRNA gene sequence analysis, strainOS17 show the high similarity with Burkholderia ambifariaAMMDT, B. diffusa AM747629T, B. tettitorii LK023503T99.8%, 99.7%, 99.6%, respectively. We investigated cell growthand antimicrobial activity according to commercial culturemedium, temperature, pH for culture optimization of strainOS17. Optimal conditions for growth and antimicrobial activityin strain OS17 were found to be: YPD medium, 35°C and pH6.5. When the strain was cultured in LB, NB, TSB, R2A mediaat 20°C and 25°C, the antimicrobial activity did not show. Culture filtrate of strain OS17 showed antimicrobial activityagainst 5 MRSA strains, Bacillus cereus, Bacillus subtilis, andFilobasidium neoformans with inhibition zones from 2 to 8mm. Optimal reaction time was 48 h in YPD medium, 100 rpmand 0.3 vvm in 2 L-scale fed-batch fermentation process forantimicrobial activity. Culture optimization of strain OS17 canbe improved on antimicrobial activity. Therefore, the antimicrobialactivity of Burkholderia sp. OS17 had potential as antibioticsfor pathogens including MRSA.
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KEYWORD
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Burkholderia sp., Staphylococcus aureus, antimicrobial activity, methicillin-resistant
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